Integration Time: This is similar to the shutter speed of a camera.There are three parameters listed in the dialog box:.Click or tap the gear to show the Spectrometer Settings dialog.The calculated column is automatically displayed on the graph. Modify the parameters and column options, if necessary. Select Insert Expression and select the appropriate equation. Modify the name, units, and displayed precision accordingly. To add a calculated column to the data set, click OK in the measurement header on the data table.To fit a function to the data, click Graph Tools, select Apply Curve Fit, and choose the appropriate curve fit.Transfer ~2 mL of the reaction mixture to a cuvette and place the cuvette in the spectrometer. The default data-collection settings collect measurements every two seconds until the user manually stops data collection.Click or tap along the line until you find the concentration value that matches your unknown's measurement. Click or tap Graph Tools and enable Interpolate. If doing Beer's law to determine the concentration of an unknown, place the unknown sample in the cuvette holder.To see the best fit line equation for the standard solutions, click Graph Tools, select Apply Curve Fit, and choose Linear.When finished, click Stop to end data collection. Repeat the previous step for the remaining samples.Enter the concentration of the sample and click or tap Keep Point. After the reading stabilizes, click Keep. Place your second sample in the cuvette slot.Your first sample should still be in the Spectrophotometer. Follow the instructions in the Choose a Wavelength dialog box.Save or export your data from the File menu.Place the sample in the Spectrophotometer and click Collect. Fill a cuvette about 3/4 full of a sample of the solution to be tested. Note: Calibration is not required for fluorescence or intensity. Align the cuvette so the clear side of the cuvette is facing the light source. If applicable, follow instructions for calibration.For complete instructions, refer to the Spectral Analysis user manual at Measurement vs. Use the Advanced Full Spectrum mode to switch between all the measurement types in the same session. You can also choose from % Transmittance, Fluorescence, Emissions, or Advanced Full Spectrum. Time-collect time-based data for a kinetics experiment.īy default, Absorbance is expanded. Concentration-conduct a Beer's law experiment. The three options for Experiment Types are: It works via USB with LabQuest 2 or LabQuest 3.Ĭonnect the sensor following the steps in the Getting Started section of this user manual. Note: This sensor does not work with the original LabQuest. You are now ready to continue your experiment. The software will identify the spectrometer and enter data-collection mode. Connect the spectrometer to the USB port.Ĭomputer: Spectral Analysis or Logger Pro 3.Note: If collecting data via USB, it is suggested you remove the battery from the spectrometer. You do not need it when taking data via USB. Click or tap Done to enter data-collection mode.The Bluetooth LED on the sensor will now glow blue (no longer flashing). Your spectrometer's ID is located near the barcode on the label. Select your Go Direct SpectroVis Plus from the list of Discovered Wireless Devices. Turn on the spectrometer by pressing the power button once.It is recommended that you not have the power supply attached while collecting data wirelessly. Disconnect the power supply before attempting to collect data.
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